HDAC7 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90622-50, BS90622-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q8WUI4(Human) Q8C2B3(Mouse) Q99P96(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, FC

All Applications: WB:1:1,000-1:2,000
FC:1:50-1:100

Background: In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Several mammalian proteins have been identified as nuclear histone acetylases, including GCN5, PCAF (p300/CBP-associated factor), p300/CBP, HAT1, and the TFIID subunit TAF II p250. Mammalian HDAC7 is a histone deacetylase that interacts with the adaptor mSin3A. The interaction of HDAC7 with mSin3A suggests the association of multiple repression complexes of transcription factors.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: HDAC7 polyclonal antibody detects endogenous levels of HDAC7 protein.

Bioworld Molecular Weight: 109/99 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of HDAC7 on different lysates using anti-HDAC7 antibody at 1/1,000 dilution.
Positive control:
Lane 1: A549
Lane 2: Human brain, Flow cytometric analysis of K562 cells with HDAC7 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody

Alternative Name: HD7, HD7a, DKFZP586J0917, FLJ99588, HD 7a, HD7, HD7a, HDAC 7, HDAC 7A, Hdac7, HDAC7, HDAC7A, Histone deacetylase 7, Histone deacetylase 7A, OTTHUMP00000202813, OTTHUMP00000202814,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified