Histone H1.0 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90637-50, BS90637-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P07305(Human) P10922(Mouse) P43278(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC/IF, IHC

All Applications: WB:1:1,000-1:5,000
ICC:1:50-1:200
IHC:1:50-1:200

Background: Eukaryotic histones are basic and water soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer; formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene, that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: Histone H1.0 polyclonal antibody detects endogenous levels of Histone H1.0 protein.

Bioworld Molecular Weight: 28 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Histone H1.0 on human lung lysates using anti-Histone H1.0 antibody at 1/1,000 dilution., ICC staining Histone H1.0 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: H1F0, H1 histone family member 0, H1(0), H10, H10, h1f0, H1FV, Histone H1'', Histone H1(0), Histone H1.0, Histone H10, Histone H5, MGC5241, N-terminally processed,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified