Fascin Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90495-50, BS90495-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q16658(Human) Q61553(Mouse) P85845(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC/IF, IHC, IP, , FC

All Applications: WB:1:500-1:1,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100

Background: Cell adhesion to extracellular matrix is an important physiological stimulus for organization of the Actin-based cytoskeleton. Adhesion to the matrix glycoprotein Thrombospondin 1 triggers the sustained formation of F-Actin microspikes that contain the Actin-bundling protein Fascin. These structures are also implicated in cell migration, which may be an important function of Thrombospondin 1 in tissue remodelling and wound repair. Fascin bundles Actin microfilaments within dynamic cellular structures such as microspikes, stress fibers and membrane ruffles. Fascin could serve as a prognostic factor for abnormal ovarian epithelial pathology and could be a novel target for the treatment of ovarian cancer. Fascin, an Actin-bundling protein, identifies dendritic cells in the blood and in tissues.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: Fascin polyclonal antibody detects endogenous levels of Fascin protein.

Bioworld Molecular Weight: 54 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Fascin on different lysates using anti-Fascin antibody at 1/1,000 dilution.
Positive control:
Lane 1: SH-SY5Y
Lane 2: Human brain
Lane 3: Rat spleen
Lane 4: Mouse testes
Lane 5: Mouse brain
Lane 6: Mouse spleen, ICC staining Fascin in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: FSCN1, FAN1, HSN, SNL, 55 kDa actin bundling protein, 55 kDa actin-bundling protein, Actin bundling protein, actin bundling protein, 55-KD, FAN 1, FAN1, Fascin 1, Fascin actin bundling protein 1, Fascin, Fascin homolog 1 actin bundling protein (Strongylocentrotus purpuratus), Fascin homolog 1, Fascin, sea urchin, homolog of, 1, Fascin1, FLJ38511, FSCN 1, FSCN1, FSCN1, HSN, p55, Singed (Drosophila) like (sea urchin fascin homolog like), Singed drosophila homolog like, Singed like (fascin homolog sea urchin) (Drosophila), Singed like (fascin homolog sea urchin), Singed like protein, Singed, drosophila, homolog of, Singed-like protein, SNL, Strongylocentrotus purpuratus antibody ,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified