Glut1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90582-50, BS90582-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P11166(Human) P17809(Mouse)

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, ICC IHC FC

All Applications: WB:1:500
ICC:1:100-1:500
IHC:1:50-1:200
FC:1:50-1:100

Background: Glucose is fundamental to the metabolism of mammalian cells. Its passage across cell membranes is mediated by a family of transporters termed glucose transporters or Gluts. In adipose and muscle tissue, insulin stimulates a rapid and dramatic increase in glucose uptake, which is largely due to the redistribution of the insulin-inducible glucose transporter, Glut4. In response to insulin, Glut4 is quickly shuttled from an intracellular storage site to the plasma membrane, where it binds glucose. In contrast, the ubiquitously expressed glucose transporter Glut1 is constitutively targeted to the plasma membrane, and shows a much less dramatic translocation in response to insulin. Glut1 and Glut4 are twelve-pass transmembrane proteins (12TM) whose carboxy-termini may dictate their cellular localization. Aberrant Glut4 expression has been suggested to contribute to such maladies as obesity and diabetes. Glut4 null mice have shown that while functional Glut4 protein is not required for maintaining normal glucose levels, it is necessary for sustained growth, normal cellular glucose, fat metabolism and prolonged longevity.

Purification and Purity: Peptide affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: Glut1 polyclonal antibody detects endogenous levels of Glut1 protein.

Bioworld Molecular Weight: 54 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Glut1 on human placenta tissue lysates using anti-Glut1 antibody at 1/1,000 dilution., ICC staining Glut1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: GLUT-1, SLC2A1, GLUT1, Choreoathetosis/spasticity episodic (paroxysmal choreoathetosis/spasticity), CSE, DYT17, DYT18, DYT9, EIG12, erythrocyte/brain, Erythrocyte/hepatoma glucose transporter, facilitated glucose transporter member 1, Glucose transporter 1, Glucose transporter type 1, Glucose transporter type 1, erythrocyte/brain, GLUT, GLUT-1, GLUT1, GLUT1DS, GLUTB, GT1, GTG1, Gtg3, GTR1, HepG2 glucose transporter, HTLVR, Human T cell leukemia virus (I and II) receptor, MGC141895, MGC141896, PED, RATGTG1, Receptor for HTLV 1 and HTLV 2, SLC2A1, Solute carrier family 2 (facilitated glucose transporter), member 1, Solute carrier family 2, Solute carrier family 2, facilitated glucose transporter member 1,

Immunogen: Peptide.

Conjugate: Unconjugated

Modification: Unmodified