Poly-L-lysine hydrochloride is a nonspecific attachment factor for cells useful in promoting cell adhesion to solid substrates by enhancing electrostatic interaction between negatively charged ions of the cell membrane and the culture surface. Poly-L-lysine hydrochloride is a strong-attraction regulator that promotes liquid-liquid phase separation (LLPS) at low concentrations but suppresses LLPS at high concentrations. Antibacterial cationic peptide..

Product information

CAS Number: 26124-78-7

Molecular Weight: 222.72

Formula: C8H19ClN4O

Chemical Name: (5S)-5-(methylamino)-6-oxoheptan-1-aminium diamine chloride

Smiles: [N].[N].[Cl-].CN[C@@H](CCCC[NH3+])C(C)=O

InChiKey: CNVBIBGCPOGMBA-CZDIJEQGSA-N

InChi: InChI=1S/C8H18N2O.ClH.2N/c1-7(11)8(10-2)5-3-4-6-9;;;/h8,10H,3-6,9H2,1-2H3;1H;;/t8-;;;/m0.../s1

Technical Data

Appearance: Solid Power

Purity: ≥98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis

Storage Condition: Dry, dark and -20 ^oC for 1 year or refer to the Certificate of Analysis.

Shelf Life: ≥12 months if stored properly.

Stock Solution Storage: 0 - 4 ^oC for 1 month or refer to the Certificate of Analysis.

Drug Formulation: To be determined

HS Tariff Code: 382200

How to use

In Vitro:

Poly-L-lysine is a food-grade antimicrobial peptide that forms complexes with proteins. Such complexes are potential carriers for targeted delivery of agents. Electrostatic potential modelling of EPL was employed to describe the interaction affinity. A three-dimensional phase boundary curve was established which divided the complexation into a nano-scale and phase separation.

References:

1. Archishman Ghosh, et al. Three archetypical classes of macromolecular regulators of protein liquid-liquid phase separation. Proc Natl Acad Sci U S A. 2019 Sep 24;116(39):19474-19483.
2. Liang Guo, et al. pH-induced structural transition during complexation and precipitation of sodium caseinate and ε-Poly-l-lysine. Int J Biol Macromol. 2020 Jul 1;154:644-653.

Products are for research use only. Not for human use.