Recombinant Mouse TROP2/TACSTD2 Protein (His Tag)(Active) Size: 100μg Catalogue Number: PKSM040435-100 Citations, Manuals and MSDS Available upon request. Abbreviation: TROP2; TACSTD2 Target Symptom: Tumor-associated calcium signal transducer 2; Tacstd2; Trop2; Cell surface glycoprotein Trop-2; TROP-2; C80403; EGP-1; GA733-1; Ly97 Target Species: Mouse Expression Host: HEK293 Cells Application: Cell culture; ELISA Fusion Tag: C-His UNIProt ID: Q8BGV3 Accession: Q8BGV3 Background: TROP-2, also referred to as tumor associated calcium signal transducer 2 (TACSTD2), GA733-1 or M1S1, is a cell surface glycoprotein highly expressed in a wide variety of epithelial cancers. In contrast, there is little or no expression of Trop-2 in adult somatic tissue. Because it is a cell surface protein that is selectively expressed in tumor cells, Trop-2 is a potential therapeutic target. The cytoplasmic tail of Trop-2 possesses potential serine and tyrosine phosphorylation sites and a phosphatidyl-inositol binding consensus sequence. Trop-2 transduces an intracellular calcium signal, are consistent with the hypothesis that it acts as a cell surface receptor and support a search for a physiological ligand. TROP2 encoding by an intronless gene was originally defined by the monoclonal antibody GA733, and is a member of a family of at least two type I membrane proteins. The other known member is GA733-2, also called EpCAM and TROP1. It has been suggested by studies that the GA733-1 gene was formed by the retroposition of the GA733-2 gene via an mRNA intermediate. Activity: Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. When 5 x 10⁴ cells/well are added to TACSTD2-coated plates (40 μg/mL, 100 μL/well), > 50% cells will adhere after 1 hour at 37℃. Sequence: Met 1-Gln 270 Purity: > 92 % as determined by reducing SDS-PAGE. Formulation: Lyophilized from sterile PBS, pH 7.4 Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. Reconstitution: Please refer to the printed manual for detailed information. Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method. Calculated MW: 29.4 kDa Observed MW: 45 kDa Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. Shipping: This product is provided as lyophilized powder which is shipped with ice packs. Research Use Only