RIN1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS75458-50, BS75458-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q13671

Host: Rabbit

Reactivity: Mouse, Rat

Applications: WB

All Applications: WB,1:500 - 1:2000

Background: Ras effector protein, which may serve as an inhibitory modulator of neuronal plasticity in aversive memory formation. Can affect Ras signaling at different levels. First, by competing with RAF1 protein for binding to activated Ras. Second, by enhancing signaling from ABL1 and ABL2, which regulate cytoskeletal remodeling. Third, by activating RAB5A, possibly by functioning as a guanine nucleotide exchange factor (GEF for RAB5A, by exchanging bound GDP for free GTP, and facilitating Ras-activated receptor endocytosis.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Extra Notes: Western blot analysis of extracts of various cell lines, using RIN1 antibody at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.

Note: For research use only, not for use in diagnostic procedure.

Bioworld Molecular Weight: 84kDa

Alternative Name: RIN1

Immunogen: Recombinant fusion protein of human RIN1(NP_004283.2).

Conjugate: Unconjugated

Modification: Unmodified