CSN5A/B Polyclonal Antibody

Catalogue Numbers: BS67286-50, BS67286-100

Sizes: 50µl, 100µl

Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Swiss-Prot: Q8LAZ7; Q9FVU9

Host: Rabbit

Reactivity: Arabidopsis thaliana

Applications: WB

All Applications: WB (1/500 - 1/2000)

Background: Probable protease subunit of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes such as photomorphogenesis and auxin and jasmonate responses. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of the SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF. In the complex, it probably acts as the catalytic center that mediates the cleavage of Nedd8 from cullins. It however has no metalloprotease activity by itself and requires the other subunits of the CSN complex (By similarity).The CSN complex is involved in repression of photomorphogenesis in darkness by regulating the activity of COP1-containing Ubl ligase complexes. The complex is also required for degradation of PSIAA6 by regulating the activity of the Ubl ligase SCF-TIR complex. Involved in CSN's deneddylation/derubylation activity.Required for the deneddylation of all cullins.Essential for the structural integrity of the CSN holocomplex.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes endogenous levels of CSN5A/B protein.

Extra Notes: Western blot analysis of CSN5A/B expression in arabidopsis thaliana (A) whole cell lysates.

Bioworld Molecular Weight: ~ 42 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: AJH1; CSN5B; COP9 signalosome complex subunit 5a; Signalosome subunit 5a; Jun activation domain-binding homolog 1; AJH2; CSN5A; COP9 signalosome complex subunit 5b; Signalosome subunit 5b; Jun activation domain-binding homolog 2

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of arabidopsis thaliana CSN5A/B. The exact sequence is proprietary.

Conjugate: Unconjugated

Modification: Unmodified