ADAM28 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS60838-50, BS60838-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9UKQ2

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200

Background: The ADAM (a disintegrin and metalloprotease) protein family, which includes over 30 membrane-anchored, glycosylated, Zn2+ dependent proteases, plays a role in cell-cell and cell-matrix interface related processes, including fertilization, muscle fusion, secretion of TNFα (tumor necrosis factor α), and modulation of the neurogenic function of Notch and Delta. The ADAM proteins possess a signal-domain, a pro-domain, a metalloprotease domain, a disintegrin domain (integrin ligand), a cysteine-rich region, an epidermal growth factorlike domain, a transmembrane domain and a cytoplasmic tail. ADAMs are expressed in a wide range of mammalian tissues and several are abundantly expressed in the male reproductive tract. ADAM28, also designated MDC-L,is more closely related to snake venom metalloproteases (SVMPs) than to other ADAM family members. ADAM28 displays a high level of expression in lymphocytes and epididymis, and functions mainly on the cell surface, where it mediates cell adhesion through its binding to integrin α4β1. The gene encoding human ADAM28 maps to chromosome 8p21.2.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: ADAM28 polyclonal antibody detects endogenous levels of ADAM28 protein.

Bioworld Molecular Weight: ~ 87 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of ADAM28 polyclonal antibody at 1:500 dilution
Lane 1: A549 whole cell lysate
Lane 2: Raw264.7 whole cell lysate, Immunohistochemistry (IHC) analyzes of ADAM28 pAb in paraffin-embedded human breast carcinoma tissue at 1:50, showing membrane and secreted staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase., Immunohistochemistry (IHC) analyzes of ADAM28 pAb in paraffin-embedded human colon carcinoma tissue at 1:50, showing membrane and secreted staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Alternative Name: Disintegrin and metalloproteinase domain-containing protein 28; ADAM 28; Epididymal metalloproteinase-like, disintegrin-like, and cysteine-rich protein II; eMDC II; Metalloproteinase-like, disintegrin-like, and cysteine-rich protein L; MDC-L; ADAM28; ADAM23; MDCL

Immunogen: A synthetic peptide corresponding to residues in Human ADAM28.

Conjugate: Unconjugated

Modification: Unmodified